Received NoA for R01 Renewal

March 24, 2023, 1:10 a.m.

Maggie-Lam

Today we are ecstatic to receive the Notice of Award for our NHLBI Alternative Splicing R01 (2R01HL141278) renewal application. We are extremely thankful to our collaborators, and we appreciate everyone in the lab who contributed to the projects and helped build the lab up since 2017. In the previous award project period, we created and refined a workflow (see JCast and Protocol) that combined RNAseq and shotgun proteomics technologies (multiomics, yay!) to confirm expression of alternative isoform proteins. We re-analyzed ~80 million mass spectra and multiple RNAseq datasets to uncover hidden isoforms in already published studies (see Cell Reports paper). We performed isotope-labeling MS and transcriptomics experiments to trace isoform switches through stages of iPSC differentiation to cardiomyocytes, and we have also determined experimentally that isoforms can be sex- and age-specific (see AJP paper).

In this new project period, we aim to continue this line of work to examine isoform changes in models of diastolic dysfunction and heart failure. We will specifically look into the molecular interactions and structural changes brought about by alternative splicing events in the heart using a series of recently developed computational and experimental tools.